DNA Transformation Into Bacteria – Biology
This quiz is about DNA Transformation Into Bacteria – Biology.
Which is true about genetic transformation?
|a. involves the insertion of a gene into an organism|
|b. results in the modification or addition of a trait|
|c. is species-specific (gene being inserted has to be of the same species as the host)|
|d. all of the above are true|
|e. a and c are true|
|f. a and b are true – correct|
Which contains the transformation procedure in proper order?
|a. 1- provide bacteria with nutrients & a short incubation period, 2- use a transformation solution of CaCl2, 3- transfer bacteria to a medium that contains ampicillin, 4- carry out heat shock|
|b. 1- use a transformation solution of CaCl2, 2- provide bacteria with nutrients & a short incubation period, 3- carry out a heat shock, 4- transfer bacteria to a medium that contains ampicillin|
|c. 1- use a transformation solution of CaCl2, 2- carry out a heat shock, 3- provide bacteria with nutrients & a short incubation period, 4- transfer bacteria to a medium that contains ampicillin – correct|
In the arabinose operon, which transcription factor is needed to bind DNA (for facilitating transcription), as well as arabinose?
|c. araC – correct|
Which 2 plates should be compared with one another to see that transformation successfully occured?
|a. + pGLO LB and +pGLO LB/amp|
|b. +pGLO LB and -pGLO LB|
|c. +pGLO LB/amp and -pGLO LB/amp – correct|
|d. +pGLO LB/amp and +pGLO LB/amp/ara|
The pGLO plasmid enables the transcription of 3 genes that metabolize arabinose.
|False – correct|
Which of the following is the correct scientific notation for the following: 973,000 ?
|a. 9.73 x 10^6|
|b. 9.73 x 10^5 – correct|
|c. 9.73 x 10^4|
Transformation efficiency” may be defined as:
|a. a measure of how effective you were in getting DNA into bacterial cells|
|b. how many non-transformed bacteria were killed by ampicillin|
|c. a representation of the transformed bacteria that express the desired traits, divided by the amount (ug) of DNA spread on the agar plate|
|d. the total number of colonies that fluoresce|
|e. a and c – correct|
In the pGLO plasmid, the ampicillin resistance gene has an inducible promoter that will turn on depending on what is in the plate bacteria is grown in.
|False – correct|
The only plate that should contain transformed bacterial cells is the +pGLO LB/amp/ara plate. This is the reason we’ll be using this plate for the pGLO 2 lab exercise