DNA Transformation Into Bacteria – Biology

DNA Transformation Into Bacteria – Biology

This quiz is about DNA Transformation Into Bacteria – Biology.


Which is true about genetic transformation?

a.  involves the insertion of a gene into an organism 
b.  results in the modification or addition of a trait
c.  is species-specific (gene being inserted has to be of the same species as the host)
d.  all of the above are true
e.  a and c are true
f.  a and b are true – correct

Which contains the transformation procedure in proper order?

a.    1- provide bacteria with nutrients & a short incubation period, 2- use a transformation solution of CaCl2, 3- transfer bacteria to a medium that contains ampicillin, 4- carry out heat shock
b.  1- use a transformation solution of CaCl2, 2- provide bacteria with nutrients & a short incubation period, 3- carry out a heat shock, 4- transfer bacteria to a medium that contains ampicillin
c.  1- use a transformation solution of CaCl2, 2- carry out a heat shock, 3- provide bacteria with nutrients & a short incubation period, 4- transfer bacteria to a medium that contains ampicillin – correct

In the arabinose operon, which transcription factor is needed to bind DNA (for facilitating transcription), as well as arabinose?

a.  araA
b.  araB
c.  araC – correct
d.  araD
e.  araE

Which 2 plates should be compared with one another to see that transformation successfully occured?

a.  + pGLO LB and +pGLO LB/amp
b.  +pGLO LB and -pGLO LB
c.  +pGLO LB/amp and -pGLO LB/amp – correct
d.  +pGLO LB/amp and +pGLO LB/amp/ara

The pGLO plasmid enables the transcription of 3 genes that metabolize arabinose.

True
False correct

Which of the following is the correct scientific notation for the following: 973,000 ?

a.  9.73 x 10^6
b.  9.73 x 10^5 – correct
c.  9.73 x 10^4

Transformation efficiency” may be defined as:

a.  a measure of how effective you were in getting DNA into bacterial cells
b.  how many non-transformed bacteria were killed by ampicillin
c.  a representation of the transformed bacteria that express the desired traits, divided by the amount (ug) of DNA spread on the agar plate
d.  the total number of colonies that fluoresce
e.  a and ccorrect

In the pGLO plasmid, the ampicillin resistance gene has an inducible promoter that will turn on depending on what is in the plate bacteria is grown in.

True
False correct

The only plate that should contain transformed bacterial cells is the +pGLO LB/amp/ara plate. This is the reason we’ll be using this plate for the pGLO 2 lab exercise

True
False -correct

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