DNA Transformation Into Bacteria – Biology
This quiz is about DNA Transformation Into Bacteria – Biology.
Which is true about genetic transformation?
| a. involves the insertion of a gene into an organism |
| b. results in the modification or addition of a trait |
| c. is species-specific (gene being inserted has to be of the same species as the host) |
| d. all of the above are true |
| e. a and c are true |
| f. a and b are true – correct |
Which contains the transformation procedure in proper order?
| a. 1- provide bacteria with nutrients & a short incubation period, 2- use a transformation solution of CaCl2, 3- transfer bacteria to a medium that contains ampicillin, 4- carry out heat shock |
| b. 1- use a transformation solution of CaCl2, 2- provide bacteria with nutrients & a short incubation period, 3- carry out a heat shock, 4- transfer bacteria to a medium that contains ampicillin |
| c. 1- use a transformation solution of CaCl2, 2- carry out a heat shock, 3- provide bacteria with nutrients & a short incubation period, 4- transfer bacteria to a medium that contains ampicillin – correct |
In the arabinose operon, which transcription factor is needed to bind DNA (for facilitating transcription), as well as arabinose?
| a. araA | |
| b. araB | |
| c. araC – correct | |
| d. araD | |
| e. araE |
Which 2 plates should be compared with one another to see that transformation successfully occured?
| a. + pGLO LB and +pGLO LB/amp |
| b. +pGLO LB and -pGLO LB |
| c. +pGLO LB/amp and -pGLO LB/amp – correct |
| d. +pGLO LB/amp and +pGLO LB/amp/ara |
The pGLO plasmid enables the transcription of 3 genes that metabolize arabinose.
| True |
| False – correct |
Which of the following is the correct scientific notation for the following: 973,000 ?
| a. 9.73 x 10^6 |
| b. 9.73 x 10^5 – correct |
| c. 9.73 x 10^4 |
Transformation efficiency” may be defined as:
| a. a measure of how effective you were in getting DNA into bacterial cells |
| b. how many non-transformed bacteria were killed by ampicillin |
| c. a representation of the transformed bacteria that express the desired traits, divided by the amount (ug) of DNA spread on the agar plate |
| d. the total number of colonies that fluoresce |
| e. a and c – correct |
In the pGLO plasmid, the ampicillin resistance gene has an inducible promoter that will turn on depending on what is in the plate bacteria is grown in.
| True |
| False – correct |
The only plate that should contain transformed bacterial cells is the +pGLO LB/amp/ara plate. This is the reason we’ll be using this plate for the pGLO 2 lab exercise
| True |
| False -correct |