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Algae & Bacteria Culture – Microbiology Exam

The key terms of Microbiology Exam include Agar, Cell, Medium, Culture, Algae, Bacteria, Negative, Bacterium, Coccus, negative stain, general medium, several-to-many flagella, lophotrichous, liquid microbial culture, Bioreactor, optical microscopy technique, mycobacteria.


Agar comes from:

bacteria.

brown algae.

golden algae.

red algae.- correct

green algae.


A bacterium is called a ________ if it’s spherical.

coccobacillus

spirillum

Coccus – correct

vibrio

bacillus


A negative stain doesn’t stick to a cell because the colored portion has a _____ charge to the cell’s ______ charge.

negative, negative – correct

positive, positive

positive, negative

negative, positive


When a medium grows many different kinds of bacteria, it is called a:

chemically-defined medium

differential medium

general medium – correct

selective medium

complex medium


Which is the term used to describe a bacterium with several-to-many flagella, all at one end of the cell?

amphitrichous

lophotrichous – correct

monotrichous

peritrichous


Place the following steps in the correct order:
1- staining       2- making a smear        3- fixing

3 -> 2 -> 1

2 -> 3 -> 1- correct

1 -> 3 -> 2

1 -> 2 -> 3


Which of the following is not an optical microscopy technique?

atomic force – correct

dark field

confocal

brightfield

fluorescent


Which of the following is used to grow the largest amount of a liquid microbial culture?

tube

flask

Bioreactor – correct

plate


Which of these stains is specific for mycobacteria?

Gram

negative

capsule

spore

acid-fast – correct


Place the steps of the Gram stain in the correct order:

1 – alcohol    2 – crystal violet   3 – safranin   4 – iodine

1 -> 2 -> 3 -> 4

4 -> 3 -> 2 -> 1

2 -> 1 -> 4 -> 3

1 -> 3 -> 2 -> 4

2 -> 4 -> 1 -> 3 – correct


Which of the following plate techniques involves thoroughly mixing the bacterial sample with liquid agar?

streak plate

pour plate – correct

spread plate


Agar comes from:

bacteria.

brown algae.

golden algae.

red algae.- correct

green algae.


A bacterium is called a ________ if it’s spherical.

coccobacillus

spirillum

Coccus – correct

vibrio

bacillus


A negative stain doesn’t stick to a cell because the colored portion has a _____ charge to the cell’s ______ charge.

negative, negative – correct

positive, positive

positive, negative

negative, positive


When a medium grows many different kinds of bacteria, it is called a:

chemically-defined medium

differential medium

general medium – correct

selective medium

complex medium