Sterile Water – Microbiology Hw

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If I took 2 ml of a culture containing 4000 cells/ml and added that to a tube containing 2 ml of sterile water, the concentration of cells in the second tube would be
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Sterile Water – Microbiology Hw

The key terms of Microbiology Homework include, Sterile Water, Cells.


If I took 2 ml of a culture containing 4000 cells/ml and added that to a tube containing 2 ml of sterile water, the concentration of cells in the second tube would be

4000 cells/ml  – correct

8000 cells

2000 cells/ml

none of these

should be 2000 cells/ml


I collect a volume of 0.1 ml of a culture. What volume of sterile water should I add to achieve a 1:10 dilution?

0.9 ml   – correct

1 ml

0.1 ml

10 ml


I have a culture estimated to contain 106 colony forming units (CFU)/ml. How many 1:10 dilutions do I need to reduce the concentration to about 102 CFU/ml?

4   – correct

6

5

3


If I plate 0.1 ml of a culture is counts as a 1:10 dilution.

True   – correct

False


What do we call the set of lenses nearest our eyes?

Oculars   – correct

eyepieces

objectives

iris diaphragm


What is a heterotroph?

a unicellular organism

a eukaryote

a sexually reproducing organism

an organism that requires organic carbon for energy and as a source of carbon  – correct


What species is Pseudomonas aeriginosa?

Pseudomonas

prokaryote

bacteria

aeriginosa  – correct


Why are bacteria hard to classify?

all of these   – correct

most cannot be cultured in the laboratory

they lack morphological diversity

there is a high degree of lateral gene transfer among bacteria


How can DNA be used to classify bacteria?

hybridization

DNA sequencing

determine G+C ratio

all of these   – correct


Classifying a bacterium is different than identifying a bacterium.

True

False


What range of number of colonies on a standard Petri plate is considered countable?

30 to 300   – correct

20 to 200

1 to 500

up to 1000


If I took 1 ml of a culture containing 7,000 cells per ml and added it to a tube containing 9 ml of sterile water, the concentration of cells in the second tube would be about

700 cells per ml   – correct

7,000 cells

7,000 cells per ml

70 cells per ml


If I took 2 ml of a culture containing 4000 cells/ml and added that to a tube containing 2 ml of sterile water, the concentration of cells in the second tube would be

4000 cells/ml  – correct

8000 cells

2000 cells/ml

none of these

should be 2000 cells/ml


I collect a volume of 0.1 ml of a culture. What volume of sterile water should I add to achieve a 1:10 dilution?

0.9 ml   – correct

1 ml

0.1 ml

10 ml


I have a culture estimated to contain 106 colony forming units (CFU)/ml. How many 1:10 dilutions do I need to reduce the concentration to about 102 CFU/ml?

4   – correct

6

5

3


If I plate 0.1 ml of a culture is counts as a 1:10 dilution.

True   – correct

False


What do we call the set of lenses nearest our eyes?

Oculars   – correct

eyepieces

objectives

iris diaphragm


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