This quiz is about DNA Enzymes And Electrophoresis – Biology
To prepare our agarose gel, how much agarose is added to a 50ml TAE buffer in an Erlenmeyer flask?
a. 0.5 g – correct
b. 0.2 g
c. 0.4 g
d. 0.1 g
In order to draw the standard curve for DNA Fingerprinting, you will need to PLOT the points, draw the LINE OF BEST FIT and your graph should be LINEAR.
False – correct
In a gel, ________________ fragments will travel FARTHER from the wells.
a. more charged
b. smaller – correct
c. less charged
DNA fragments are ________________ charged; when they are placed in an electric field, they will be drawn towards the _______________ pole.
a. negatively, positive – correct
b. positively, negative
c. neutrally, positive
d. negatively, neutral
How much loading dye is added to each of your DNA samples?
a. 5 ul – correct
b. 4 ul
c. 7 ul
d. 6 ul
In the image of a gel, the lane containing our KB DNA ladder will be used to construct the Standard Curve.
True – correct
___________________ is the specific procedure that will be used to determine how well DNA was restricted by restriction enzymes and which suspect’s DNA sample is similar to the DNA found at the crime scene.
Agarose Gel Electrophoresis
Electrophoresis – correct
Running a gel
The distance from the well to a band is properly measured:
a. None of the above is correct
b. From the top of the gel to the center of the well
c. From the bottom of the well to the center of the band
d. From the top of the gel to the bottom of the band
e. From the bottom of the well to the bottom of the band – correct
Restriction enzymes will digest (hydrolize) DNA at specific areas known as reaction sites.