Lecture Test 2 Cell And Bacteria Culture Microbiology
This lecture is about Cell And Bacteria culture Microbiology.
Which is the term used to describe a bacterium with several-to-many flagella, all at one end of the cell?
lophotrichous – correct
Place the following steps in the correct order:
1- staining 2- making a smear 3- fixing
3 -> 2 -> 1
2 -> 3 -> 1- correct
1 -> 3 -> 2
1 -> 2 -> 3
Which of the following is not an optical microscopy technique?
atomic force – correct
Which of the following is used to grow the largest amount of a liquid microbial culture?
Bioreactor – correct
Which of these stains is specific for mycobacteria?
acid-fast – correct
Place the steps of the Gram stain in the correct order:
1 – alcohol 2 – crystal violet 3 – safranin 4 – iodine
1 -> 2 -> 3 -> 4
4 -> 3 -> 2 -> 1
2 -> 1 -> 4 -> 3
1 -> 3 -> 2 -> 4
2 -> 4 -> 1 -> 3 – correct
Which of the following plate techniques involves thoroughly mixing the bacterial sample with liquid agar?
pour plate – correct
Agar comes from:
red algae.- correct
A bacterium is called a ________ if it’s spherical.
Coccus – correct
A negative stain doesn’t stick to a cell because the colored portion has a _____ charge to the cell’s ______ charge.
negative, negative – correct
When a medium grows many different kinds of bacteria, it is called a:
general medium – correct
Which of the following tools is used to transfer a very small amount, about 10 microliters (ul) or less, of a liquid culture to another container?
small graduated cylinder
syringe and needle
Pipet – correct
Explanation Note the word "about", that means the measurement is not precisely 10 ul. Also a pipet can't measure that small an amount, one needs to use a micropipet/micropipettor which is a different tool.
A major difference between a transmission electron microscope (TEM) and a scanning electron microscope (SEM) is in the:
number of electrons used
colors of the images produced
level of technical skill of the user
type of lenses
interaction of electron beam and the specimens – correct
Using a microscope, you observe a microbe moving toward a food source. This is an example of:
responsiveness/irritability – correct
evolution of the lineage
A bacterial colony on an streak plate comes from:
one cell in the original sample – correct
a small number of cells in the original sample
a large number of cells in the original sample
A culture medium on which the O:157:H7 strain of E. coli is black and all others are white is called a(n):
differential medium and enrichment culture
differential medium – correct
selective medium and differential medium
A(n) ______ stain sticks to cells because of its opposite charge.
Basic – correct
Three different culture media
|Medium A||Medium B||Medium C|
|KH2PO4||Na2HPO4||peptone (protein extract)|
In Table 6.1, which medium (media) is/are chemically defined, a.k.a. synthetic?
B & C
A & B
A – correct
Which of the following is not a distinguishing characteristic of prokaryotic cells?
They have a single circular chromosome.
Their DNA is not associated with many proteins.
They often have cell walls often containing peptidoglycan.
They have a plasma/cell membrane – correct
They lack membrane-enclosed organelles.
The term for a line-like aggregation of prokaryotic cells is:
Strepto – correct