Cell And Bacteria Culture Microbiology

Lecture Test 2 Cell And Bacteria Culture Microbiology

This lecture is about Cell And Bacteria culture Microbiology.


Which is the term used to describe a bacterium with several-to-many flagella, all at one end of the cell?

amphitrichous

lophotrichous – correct

monotrichous

peritrichous


Place the following steps in the correct order:
1- staining       2- making a smear        3- fixing

3 -> 2 -> 1

2 -> 3 -> 1- correct

1 -> 3 -> 2

1 -> 2 -> 3


Which of the following is not an optical microscopy technique?

atomic force – correct

dark field

confocal

brightfield

fluorescent


Which of the following is used to grow the largest amount of a liquid microbial culture?

tube

flask

Bioreactor – correct

plate


Which of these stains is specific for mycobacteria?

Gram

negative

capsule

spore

acid-fast – correct


Place the steps of the Gram stain in the correct order:

1 – alcohol    2 – crystal violet   3 – safranin   4 – iodine

1 -> 2 -> 3 -> 4

4 -> 3 -> 2 -> 1

2 -> 1 -> 4 -> 3

1 -> 3 -> 2 -> 4

2 -> 4 -> 1 -> 3 – correct


Which of the following plate techniques involves thoroughly mixing the bacterial sample with liquid agar?

streak plate

pour plate – correct

spread plate


Agar comes from:

bacteria.

brown algae.

golden algae.

red algae.- correct

green algae.


A bacterium is called a ________ if it’s spherical.

coccobacillus

spirillum

Coccus – correct

vibrio

bacillus


A negative stain doesn’t stick to a cell because the colored portion has a _____ charge to the cell’s ______ charge.

negative, negative – correct

positive, positive

positive, negative

negative, positive


When a medium grows many different kinds of bacteria, it is called a:

chemically-defined medium

differential medium

general medium – correct

selective medium

complex medium


Which of the following tools is used to transfer a very small amount, about 10 microliters (ul) or less, of a liquid culture to another container?

small graduated cylinder

syringe and needle

loop

needle

Pipet – correct

Explanation
Note the word "about", that means the measurement is not precisely 10 ul. Also a pipet can't measure that small an amount, one needs to use a micropipet/micropipettor which is a different tool.

A major difference between a transmission electron microscope (TEM) and a scanning electron microscope (SEM) is in the:

number of electrons used

colors of the images produced

level of technical skill of the user

type of lenses

interaction of electron beam and the specimens – correct


Using a microscope, you observe a microbe moving toward a food source. This is an example of:

responsiveness/irritability – correct

reproduction

growth

cellular structure

evolution of the lineage


A bacterial colony on an streak plate comes from:

one cell in the original sample – correct

a small number of cells in the original sample

a large number of cells in the original sample


A culture medium on which the O:157:H7 strain of E. coli is black and all others are white is called a(n):

selective medium

enrichment culture

differential medium and enrichment culture

differential medium – correct

selective medium and differential medium


A(n) ______ stain sticks to cells because of its opposite charge.

negative

neutral

Basic – correct

acidic


Table 6.1
Three different culture media

Medium AMedium BMedium C
Na2HPO4yeast extractglucose
KH2PO4Na2HPO4peptone (protein extract)
MgSO4KH2PO4(NH4)2SO4
CaCl2MgSO4KH2PO4
NaHCO3(NH4)2SO4Na2HPO4

In Table 6.1, which medium (media) is/are chemically defined, a.k.a. synthetic?

C

B & C

A & B

B

A – correct


Which of the following is not a distinguishing characteristic of prokaryotic cells?

They have a single circular chromosome.

Their DNA is not associated with many proteins.

They often have cell walls often containing peptidoglycan.

They have a plasma/cell membrane – correct

They lack membrane-enclosed organelles.


The term for a line-like aggregation of prokaryotic cells is:

Strepto – correct

sarcina

tetrad

staphylo-

diplo-


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